Quantitative RT-PCR:Article Title: Long noncoding RNA RUSC1-AS1 promotes tumorigenesis in cervical cancer by acting as a competing endogenous RNA of microRNA-744 and consequently increasing Bcl-2 expression
Article Snippet: The tissue samples were snap-frozen in liquid nitrogen after the surgical resection and then transferred to a − 80°C freezer for storage until RNA extraction.The tissue samples were snap-frozen in liquid nitrogen after the surgical resection and then transferred to a − 80°C freezer for storage until RNA extraction.. Cell lines and culture conditions A normal human cervix epithelial cell line (Ect1/E6E7) was purchased from the American Type Culture Collection (Manassas, VA, USA).. Four human cervical cancer cell lines (HeLa, C-33A, SiHa, and CaSki) were ordered from the Shanghai Institute of Biochemistry and Cell Biology (Shanghai, China).Four human cervical cancer cell lines (HeLa, C-33A, SiHa, and CaSki) were ordered from the Shanghai Institute of Biochemistry and Cell Biology (Shanghai, China).
Article Title: Long noncoding RNA RUSC1-AS1 promotes tumorigenesis in cervical cancer by acting as a competing endogenous RNA of microRNA-744 and consequently increasing Bcl-2 expression
Article Snippet: A normal human cervix epithelial cell line (Ect1/E6E7) was purchased from the American Type Culture Collection (Manassas, VA, USA).. Four human cervical cancer cell lines (HeLa, C-33A, SiHa, and CaSki) were ordered from the Shanghai Institute of Biochemistry and Cell Biology (Shanghai, China).Four human cervical cancer cell lines (HeLa, C-33A, SiHa, and CaSki) were ordered from the Shanghai Institute of Biochemistry and Cell Biology (Shanghai, China).
Article Title: Long Noncoding RNA FOXD2-AS1 Promotes the Malignancy of Cervical Cancer by Sponging MicroRNA-760 and Upregulating Hepatoma-Derived Growth Factor
Article Snippet: A normal human cervix epithelial cell line (Ect1/E6E7) was acquired from the American Type Culture Collection (Manassas, VA, USA).. Four human cervical cancer cell lines (HeLa, C-33A, SiHa, and CaSki) were ordered from the Shanghai Institute of Biochemistry and Cell Biology (Shanghai, China).Four human cervical cancer cell lines (HeLa, C-33A, SiHa, and CaSki) were ordered from the Shanghai Institute of Biochemistry and Cell Biology (Shanghai, China).
Article Title: Long Noncoding RNA SNHG7, a Molecular Sponge for microRNA-485, Promotes the Aggressive Behavior of Cervical Cancer by Regulating PAK4
Article Snippet: Four human cervical cancer cell lines (HeLa, SiHa, C-33A, and CaSki) were obtained from the Shanghai Institute of Cell Biology, the Chinese Academy of Sciences (Shanghai, China).Four human cervical cancer cell lines (HeLa, SiHa, C-33A, and CaSki) were obtained from the Shanghai Institute of Cell Biology, the Chinese Academy of Sciences (Shanghai, China).. Ect1/E6E7, a normal human cervix epithelial cell line, was purchased from the American Type Culture Collection (Manassas, VA, USA).. All cells were grown in Dulbecco’s modified Eagle’s medium (DMEM; Gibco; Thermo Fisher Scientific, Inc., Waltham, MA, USA) supplemented with 10% (v/v) of fetal bovine serum (FBS; Gibco; Thermo Fisher Scientific, Inc.) and 1% (v/v) of a solution of antibiotics (final concentration 100 U/mL penicillin and 100 μg/mL streptomycin; Gibco; Thermo Fisher Scientific, Inc.).All cells were grown in Dulbecco’s modified Eagle’s medium (DMEM; Gibco; Thermo Fisher Scientific, Inc., Waltham, MA, USA) supplemented with 10% (v/v) of fetal bovine serum (FBS; Gibco; Thermo Fisher Scientific, Inc.) and 1% (v/v) of a solution of antibiotics (final concentration 100 U/mL penicillin and 100 μg/mL streptomycin; Gibco; Thermo Fisher Scientific,..
Article Title: Long Noncoding RNA ZFPM2-AS1 Enhances the Malignancy of Cervical Cancer by Functioning as a Molecular Sponge of microRNA-511-3p and Consequently Increasing FGFR2 Expression
Article Snippet: Four cervical cancer cell lines, HeLa, SiHa, C-33A, and CaSki, were purchased from the Institute of Biochemistry and Cell Biology of the Chinese Academy of Sciences (Shanghai, China), and a normal human cervix epithelial cell line (Ect1/E6E7) from the American Type Culture Collection (Manassas, VA, USA).. All the aforementioned cell lines were maintained in Dulbecco’s modified Eagle’s medium (DMEM; Gibco; Thermo Fisher Scientific, Inc., Waltham, MA, USA) containing 10% of fetal bovine serum (FBS; Gibco; Thermo Fisher Scientific, Inc.), 100 U/mL penicillin, and 100 μg/mL streptomycin (Gibco; Thermo Fisher Scientific, Inc.) at 37 °C in a humidified atmosphere supplied with 5% of CO 2 .All the aforementioned cell lines were maintained in Dulbecco’s modified Eagle’s medium (DMEM; Gibco; Thermo Fisher Scientific, Inc., Waltham, MA, USA) containing 10% of fetal bovine serum (FBS; Gibco; Thermo Fisher Scientific, Inc.), 100 U/mL penicillin, and 100 μg/mL streptomycin (Gibco;..
Article Title: <p>Long Noncoding RNA SNHG7, a Molecular Sponge for microRNA-485, Promotes the Aggressive Behavior of Cervical Cancer by Regulating PAK4</p>
Article Snippet: Cell Lines Four human cervical cancer cell lines (HeLa, SiHa, C-33A, and CaSki) were obtained from the Shanghai Institute of Cell Biology, the Chinese Academy of Sciences (Shanghai, China).Cell Lines Four human cervical cancer cell lines (HeLa, SiHa, C-33A, and CaSki) were obtained from the Shanghai Institute of Cell Biology, the Chinese Academy of Sciences (Shanghai, China).. Ect1/E6E7, a normal human cervix epithelial cell line, was purchased from the American Type Culture Collection (Manassas, VA, USA).. All cells were grown in Dulbecco’s modified Eagle’s medium (DMEM; Gibco; Thermo Fisher Scientific, Inc., Waltham, MA, USA) supplemented with 10% (v/v) of fetal bovine serum (FBS; Gibco; Thermo Fisher Scientific, Inc.) and 1% (v/v) of a solution of antibiotics (final concentration 100 U/mL penicillin and 100 μg/mL streptomycin; Gibco; Thermo Fisher Scientific, Inc.).All cells were grown in Dulbecco’s modified Eagle’s medium (DMEM; Gibco; Thermo Fisher Scientific, Inc., Waltham, MA, USA) supplemented with 10% (v/v) of fetal bovine serum (FBS; Gibco; Thermo Fisher Scientific, Inc.) and 1% (v/v) of a solution of antibiotics (final concentration 100 U/mL penicillin and 100 μg/mL streptomycin; Gibco; Thermo Fisher Scientific,..
Article Title: <p>Long Noncoding RNA <em>ZFPM2-AS1</em> Enhances the Malignancy of Cervical Cancer by Functioning as a Molecular Sponge of microRNA-511-3p and Consequently Increasing FGFR2 Expression</p>
Article Snippet: The obtained tissue specimens were quickly frozen in liquid nitrogen and stored in liquid nitrogen until analysis.The obtained tissue specimens were quickly frozen in liquid nitrogen and stored in liquid nitrogen until analysis.. Cell Lines Four cervical cancer cell lines, HeLa, SiHa, C-33A, and CaSki, were purchased from the Institute of Biochemistry and Cell Biology of the Chinese Academy of Sciences (Shanghai, China), and a normal human cervix epithelial cell line (Ect1/E6E7) from the American Type Culture Collection (Manassas, VA, USA).. All the aforementioned cell lines were maintained in Dulbecco’s modified Eagle’s medium (DMEM; Gibco; Thermo Fisher Scientific, Inc., Waltham, MA, USA) containing 10% of fetal bovine serum (FBS; Gibco; Thermo Fisher Scientific, Inc.), 100 U/mL penicillin, and 100 μg/mL streptomycin (Gibco; Thermo Fisher Scientific, Inc.) at 37 °C in a humidified atmosphere supplied with 5% of CO2.All the aforementioned cell lines were maintained in Dulbecco’s modified Eagle’s medium (DMEM; Gibco; Thermo Fisher Scientific, Inc., Waltham, MA, USA) containing 10% of fetal bovine serum (FBS; Gibco; Thermo Fisher Scientific, Inc.), 100 U/mL penicillin, and 100 μg/mL streptomycin..
Expressing:Article Title: Long noncoding RNA RUSC1-AS1 promotes tumorigenesis in cervical cancer by acting as a competing endogenous RNA of microRNA-744 and consequently increasing Bcl-2 expression
Article Snippet: The tissue samples were snap-frozen in liquid nitrogen after the surgical resection and then transferred to a − 80°C freezer for storage until RNA extraction.The tissue samples were snap-frozen in liquid nitrogen after the surgical resection and then transferred to a − 80°C freezer for storage until RNA extraction.. Cell lines and culture conditions A normal human cervix epithelial cell line (Ect1/E6E7) was purchased from the American Type Culture Collection (Manassas, VA, USA).. Four human cervical cancer cell lines (HeLa, C-33A, SiHa, and CaSki) were ordered from the Shanghai Institute of Biochemistry and Cell Biology (Shanghai, China).Four human cervical cancer cell lines (HeLa, C-33A, SiHa, and CaSki) were ordered from the Shanghai Institute of Biochemistry and Cell Biology (Shanghai, China).
Article Title: Long noncoding RNA RUSC1-AS1 promotes tumorigenesis in cervical cancer by acting as a competing endogenous RNA of microRNA-744 and consequently increasing Bcl-2 expression
Article Snippet: A normal human cervix epithelial cell line (Ect1/E6E7) was purchased from the American Type Culture Collection (Manassas, VA, USA).. Four human cervical cancer cell lines (HeLa, C-33A, SiHa, and CaSki) were ordered from the Shanghai Institute of Biochemistry and Cell Biology (Shanghai, China).Four human cervical cancer cell lines (HeLa, C-33A, SiHa, and CaSki) were ordered from the Shanghai Institute of Biochemistry and Cell Biology (Shanghai, China).
Article Title: Long Noncoding RNA FOXD2-AS1 Promotes the Malignancy of Cervical Cancer by Sponging MicroRNA-760 and Upregulating Hepatoma-Derived Growth Factor
Article Snippet: A normal human cervix epithelial cell line (Ect1/E6E7) was acquired from the American Type Culture Collection (Manassas, VA, USA).. Four human cervical cancer cell lines (HeLa, C-33A, SiHa, and CaSki) were ordered from the Shanghai Institute of Biochemistry and Cell Biology (Shanghai, China).Four human cervical cancer cell lines (HeLa, C-33A, SiHa, and CaSki) were ordered from the Shanghai Institute of Biochemistry and Cell Biology (Shanghai, China).
Article Title: Long Noncoding RNA SNHG7, a Molecular Sponge for microRNA-485, Promotes the Aggressive Behavior of Cervical Cancer by Regulating PAK4
Article Snippet: Four human cervical cancer cell lines (HeLa, SiHa, C-33A, and CaSki) were obtained from the Shanghai Institute of Cell Biology, the Chinese Academy of Sciences (Shanghai, China).Four human cervical cancer cell lines (HeLa, SiHa, C-33A, and CaSki) were obtained from the Shanghai Institute of Cell Biology, the Chinese Academy of Sciences (Shanghai, China).. Ect1/E6E7, a normal human cervix epithelial cell line, was purchased from the American Type Culture Collection (Manassas, VA, USA).. All cells were grown in Dulbecco’s modified Eagle’s medium (DMEM; Gibco; Thermo Fisher Scientific, Inc., Waltham, MA, USA) supplemented with 10% (v/v) of fetal bovine serum (FBS; Gibco; Thermo Fisher Scientific, Inc.) and 1% (v/v) of a solution of antibiotics (final concentration 100 U/mL penicillin and 100 μg/mL streptomycin; Gibco; Thermo Fisher Scientific, Inc.).All cells were grown in Dulbecco’s modified Eagle’s medium (DMEM; Gibco; Thermo Fisher Scientific, Inc., Waltham, MA, USA) supplemented with 10% (v/v) of fetal bovine serum (FBS; Gibco; Thermo Fisher Scientific, Inc.) and 1% (v/v) of a solution of antibiotics (final concentration 100 U/mL penicillin and 100 μg/mL streptomycin; Gibco; Thermo Fisher Scientific,..
Article Title: Long Noncoding RNA ZFPM2-AS1 Enhances the Malignancy of Cervical Cancer by Functioning as a Molecular Sponge of microRNA-511-3p and Consequently Increasing FGFR2 Expression
Article Snippet: Four cervical cancer cell lines, HeLa, SiHa, C-33A, and CaSki, were purchased from the Institute of Biochemistry and Cell Biology of the Chinese Academy of Sciences (Shanghai, China), and a normal human cervix epithelial cell line (Ect1/E6E7) from the American Type Culture Collection (Manassas, VA, USA).. All the aforementioned cell lines were maintained in Dulbecco’s modified Eagle’s medium (DMEM; Gibco; Thermo Fisher Scientific, Inc., Waltham, MA, USA) containing 10% of fetal bovine serum (FBS; Gibco; Thermo Fisher Scientific, Inc.), 100 U/mL penicillin, and 100 μg/mL streptomycin (Gibco; Thermo Fisher Scientific, Inc.) at 37 °C in a humidified atmosphere supplied with 5% of CO 2 .All the aforementioned cell lines were maintained in Dulbecco’s modified Eagle’s medium (DMEM; Gibco; Thermo Fisher Scientific, Inc., Waltham, MA, USA) containing 10% of fetal bovine serum (FBS; Gibco; Thermo Fisher Scientific, Inc.), 100 U/mL penicillin, and 100 μg/mL streptomycin (Gibco;..
Article Title: <p>Long Noncoding RNA SNHG7, a Molecular Sponge for microRNA-485, Promotes the Aggressive Behavior of Cervical Cancer by Regulating PAK4</p>
Article Snippet: Cell Lines Four human cervical cancer cell lines (HeLa, SiHa, C-33A, and CaSki) were obtained from the Shanghai Institute of Cell Biology, the Chinese Academy of Sciences (Shanghai, China).Cell Lines Four human cervical cancer cell lines (HeLa, SiHa, C-33A, and CaSki) were obtained from the Shanghai Institute of Cell Biology, the Chinese Academy of Sciences (Shanghai, China).. Ect1/E6E7, a normal human cervix epithelial cell line, was purchased from the American Type Culture Collection (Manassas, VA, USA).. All cells were grown in Dulbecco’s modified Eagle’s medium (DMEM; Gibco; Thermo Fisher Scientific, Inc., Waltham, MA, USA) supplemented with 10% (v/v) of fetal bovine serum (FBS; Gibco; Thermo Fisher Scientific, Inc.) and 1% (v/v) of a solution of antibiotics (final concentration 100 U/mL penicillin and 100 μg/mL streptomycin; Gibco; Thermo Fisher Scientific, Inc.).All cells were grown in Dulbecco’s modified Eagle’s medium (DMEM; Gibco; Thermo Fisher Scientific, Inc., Waltham, MA, USA) supplemented with 10% (v/v) of fetal bovine serum (FBS; Gibco; Thermo Fisher Scientific, Inc.) and 1% (v/v) of a solution of antibiotics (final concentration 100 U/mL penicillin and 100 μg/mL streptomycin; Gibco; Thermo Fisher Scientific,..
Article Title: <p>Long Noncoding RNA <em>ZFPM2-AS1</em> Enhances the Malignancy of Cervical Cancer by Functioning as a Molecular Sponge of microRNA-511-3p and Consequently Increasing FGFR2 Expression</p>
Article Snippet: The obtained tissue specimens were quickly frozen in liquid nitrogen and stored in liquid nitrogen until analysis.The obtained tissue specimens were quickly frozen in liquid nitrogen and stored in liquid nitrogen until analysis.. Cell Lines Four cervical cancer cell lines, HeLa, SiHa, C-33A, and CaSki, were purchased from the Institute of Biochemistry and Cell Biology of the Chinese Academy of Sciences (Shanghai, China), and a normal human cervix epithelial cell line (Ect1/E6E7) from the American Type Culture Collection (Manassas, VA, USA).. All the aforementioned cell lines were maintained in Dulbecco’s modified Eagle’s medium (DMEM; Gibco; Thermo Fisher Scientific, Inc., Waltham, MA, USA) containing 10% of fetal bovine serum (FBS; Gibco; Thermo Fisher Scientific, Inc.), 100 U/mL penicillin, and 100 μg/mL streptomycin (Gibco; Thermo Fisher Scientific, Inc.) at 37 °C in a humidified atmosphere supplied with 5% of CO2.All the aforementioned cell lines were maintained in Dulbecco’s modified Eagle’s medium (DMEM; Gibco; Thermo Fisher Scientific, Inc., Waltham, MA, USA) containing 10% of fetal bovine serum (FBS; Gibco; Thermo Fisher Scientific, Inc.), 100 U/mL penicillin, and 100 μg/mL streptomycin..
Over Expression:Article Title: Long noncoding RNA RUSC1-AS1 promotes tumorigenesis in cervical cancer by acting as a competing endogenous RNA of microRNA-744 and consequently increasing Bcl-2 expression
Article Snippet: The tissue samples were snap-frozen in liquid nitrogen after the surgical resection and then transferred to a − 80°C freezer for storage until RNA extraction.The tissue samples were snap-frozen in liquid nitrogen after the surgical resection and then transferred to a − 80°C freezer for storage until RNA extraction.. Cell lines and culture conditions A normal human cervix epithelial cell line (Ect1/E6E7) was purchased from the American Type Culture Collection (Manassas, VA, USA).. Four human cervical cancer cell lines (HeLa, C-33A, SiHa, and CaSki) were ordered from the Shanghai Institute of Biochemistry and Cell Biology (Shanghai, China).Four human cervical cancer cell lines (HeLa, C-33A, SiHa, and CaSki) were ordered from the Shanghai Institute of Biochemistry and Cell Biology (Shanghai, China).
Article Title: Long noncoding RNA RUSC1-AS1 promotes tumorigenesis in cervical cancer by acting as a competing endogenous RNA of microRNA-744 and consequently increasing Bcl-2 expression
Article Snippet: A normal human cervix epithelial cell line (Ect1/E6E7) was purchased from the American Type Culture Collection (Manassas, VA, USA).. Four human cervical cancer cell lines (HeLa, C-33A, SiHa, and CaSki) were ordered from the Shanghai Institute of Biochemistry and Cell Biology (Shanghai, China).Four human cervical cancer cell lines (HeLa, C-33A, SiHa, and CaSki) were ordered from the Shanghai Institute of Biochemistry and Cell Biology (Shanghai, China).
Article Title: Long Noncoding RNA FOXD2-AS1 Promotes the Malignancy of Cervical Cancer by Sponging MicroRNA-760 and Upregulating Hepatoma-Derived Growth Factor
Article Snippet: A normal human cervix epithelial cell line (Ect1/E6E7) was acquired from the American Type Culture Collection (Manassas, VA, USA).. Four human cervical cancer cell lines (HeLa, C-33A, SiHa, and CaSki) were ordered from the Shanghai Institute of Biochemistry and Cell Biology (Shanghai, China).Four human cervical cancer cell lines (HeLa, C-33A, SiHa, and CaSki) were ordered from the Shanghai Institute of Biochemistry and Cell Biology (Shanghai, China).
Article Title: Long Noncoding RNA SNHG7, a Molecular Sponge for microRNA-485, Promotes the Aggressive Behavior of Cervical Cancer by Regulating PAK4
Article Snippet: Four human cervical cancer cell lines (HeLa, SiHa, C-33A, and CaSki) were obtained from the Shanghai Institute of Cell Biology, the Chinese Academy of Sciences (Shanghai, China).Four human cervical cancer cell lines (HeLa, SiHa, C-33A, and CaSki) were obtained from the Shanghai Institute of Cell Biology, the Chinese Academy of Sciences (Shanghai, China).. Ect1/E6E7, a normal human cervix epithelial cell line, was purchased from the American Type Culture Collection (Manassas, VA, USA).. All cells were grown in Dulbecco’s modified Eagle’s medium (DMEM; Gibco; Thermo Fisher Scientific, Inc., Waltham, MA, USA) supplemented with 10% (v/v) of fetal bovine serum (FBS; Gibco; Thermo Fisher Scientific, Inc.) and 1% (v/v) of a solution of antibiotics (final concentration 100 U/mL penicillin and 100 μg/mL streptomycin; Gibco; Thermo Fisher Scientific, Inc.).All cells were grown in Dulbecco’s modified Eagle’s medium (DMEM; Gibco; Thermo Fisher Scientific, Inc., Waltham, MA, USA) supplemented with 10% (v/v) of fetal bovine serum (FBS; Gibco; Thermo Fisher Scientific, Inc.) and 1% (v/v) of a solution of antibiotics (final concentration 100 U/mL penicillin and 100 μg/mL streptomycin; Gibco; Thermo Fisher Scientific,..
Article Title: Long Noncoding RNA ZFPM2-AS1 Enhances the Malignancy of Cervical Cancer by Functioning as a Molecular Sponge of microRNA-511-3p and Consequently Increasing FGFR2 Expression
Article Snippet: Four cervical cancer cell lines, HeLa, SiHa, C-33A, and CaSki, were purchased from the Institute of Biochemistry and Cell Biology of the Chinese Academy of Sciences (Shanghai, China), and a normal human cervix epithelial cell line (Ect1/E6E7) from the American Type Culture Collection (Manassas, VA, USA).. All the aforementioned cell lines were maintained in Dulbecco’s modified Eagle’s medium (DMEM; Gibco; Thermo Fisher Scientific, Inc., Waltham, MA, USA) containing 10% of fetal bovine serum (FBS; Gibco; Thermo Fisher Scientific, Inc.), 100 U/mL penicillin, and 100 μg/mL streptomycin (Gibco; Thermo Fisher Scientific, Inc.) at 37 °C in a humidified atmosphere supplied with 5% of CO 2 .All the aforementioned cell lines were maintained in Dulbecco’s modified Eagle’s medium (DMEM; Gibco; Thermo Fisher Scientific, Inc., Waltham, MA, USA) containing 10% of fetal bovine serum (FBS; Gibco; Thermo Fisher Scientific, Inc.), 100 U/mL penicillin, and 100 μg/mL streptomycin (Gibco;..
Article Title: <p>Long Noncoding RNA SNHG7, a Molecular Sponge for microRNA-485, Promotes the Aggressive Behavior of Cervical Cancer by Regulating PAK4</p>
Article Snippet: Cell Lines Four human cervical cancer cell lines (HeLa, SiHa, C-33A, and CaSki) were obtained from the Shanghai Institute of Cell Biology, the Chinese Academy of Sciences (Shanghai, China).Cell Lines Four human cervical cancer cell lines (HeLa, SiHa, C-33A, and CaSki) were obtained from the Shanghai Institute of Cell Biology, the Chinese Academy of Sciences (Shanghai, China).. Ect1/E6E7, a normal human cervix epithelial cell line, was purchased from the American Type Culture Collection (Manassas, VA, USA).. All cells were grown in Dulbecco’s modified Eagle’s medium (DMEM; Gibco; Thermo Fisher Scientific, Inc., Waltham, MA, USA) supplemented with 10% (v/v) of fetal bovine serum (FBS; Gibco; Thermo Fisher Scientific, Inc.) and 1% (v/v) of a solution of antibiotics (final concentration 100 U/mL penicillin and 100 μg/mL streptomycin; Gibco; Thermo Fisher Scientific, Inc.).All cells were grown in Dulbecco’s modified Eagle’s medium (DMEM; Gibco; Thermo Fisher Scientific, Inc., Waltham, MA, USA) supplemented with 10% (v/v) of fetal bovine serum (FBS; Gibco; Thermo Fisher Scientific, Inc.) and 1% (v/v) of a solution of antibiotics (final concentration 100 U/mL penicillin and 100 μg/mL streptomycin; Gibco; Thermo Fisher Scientific,..
Article Title: <p>Long Noncoding RNA <em>ZFPM2-AS1</em> Enhances the Malignancy of Cervical Cancer by Functioning as a Molecular Sponge of microRNA-511-3p and Consequently Increasing FGFR2 Expression</p>
Article Snippet: The obtained tissue specimens were quickly frozen in liquid nitrogen and stored in liquid nitrogen until analysis.The obtained tissue specimens were quickly frozen in liquid nitrogen and stored in liquid nitrogen until analysis.. Cell Lines Four cervical cancer cell lines, HeLa, SiHa, C-33A, and CaSki, were purchased from the Institute of Biochemistry and Cell Biology of the Chinese Academy of Sciences (Shanghai, China), and a normal human cervix epithelial cell line (Ect1/E6E7) from the American Type Culture Collection (Manassas, VA, USA).. All the aforementioned cell lines were maintained in Dulbecco’s modified Eagle’s medium (DMEM; Gibco; Thermo Fisher Scientific, Inc., Waltham, MA, USA) containing 10% of fetal bovine serum (FBS; Gibco; Thermo Fisher Scientific, Inc.), 100 U/mL penicillin, and 100 μg/mL streptomycin (Gibco; Thermo Fisher Scientific, Inc.) at 37 °C in a humidified atmosphere supplied with 5% of CO2.All the aforementioned cell lines were maintained in Dulbecco’s modified Eagle’s medium (DMEM; Gibco; Thermo Fisher Scientific, Inc., Waltham, MA, USA) containing 10% of fetal bovine serum (FBS; Gibco; Thermo Fisher Scientific, Inc.), 100 U/mL penicillin, and 100 μg/mL streptomycin..
Control:Article Title: Long noncoding RNA RUSC1-AS1 promotes tumorigenesis in cervical cancer by acting as a competing endogenous RNA of microRNA-744 and consequently increasing Bcl-2 expression
Article Snippet: The tissue samples were snap-frozen in liquid nitrogen after the surgical resection and then transferred to a − 80°C freezer for storage until RNA extraction.The tissue samples were snap-frozen in liquid nitrogen after the surgical resection and then transferred to a − 80°C freezer for storage until RNA extraction.. Cell lines and culture conditions A normal human cervix epithelial cell line (Ect1/E6E7) was purchased from the American Type Culture Collection (Manassas, VA, USA).. Four human cervical cancer cell lines (HeLa, C-33A, SiHa, and CaSki) were ordered from the Shanghai Institute of Biochemistry and Cell Biology (Shanghai, China).Four human cervical cancer cell lines (HeLa, C-33A, SiHa, and CaSki) were ordered from the Shanghai Institute of Biochemistry and Cell Biology (Shanghai, China).
Article Title: Long noncoding RNA RUSC1-AS1 promotes tumorigenesis in cervical cancer by acting as a competing endogenous RNA of microRNA-744 and consequently increasing Bcl-2 expression
Article Snippet: A normal human cervix epithelial cell line (Ect1/E6E7) was purchased from the American Type Culture Collection (Manassas, VA, USA).. Four human cervical cancer cell lines (HeLa, C-33A, SiHa, and CaSki) were ordered from the Shanghai Institute of Biochemistry and Cell Biology (Shanghai, China).Four human cervical cancer cell lines (HeLa, C-33A, SiHa, and CaSki) were ordered from the Shanghai Institute of Biochemistry and Cell Biology (Shanghai, China).
Article Title: Long Noncoding RNA FOXD2-AS1 Promotes the Malignancy of Cervical Cancer by Sponging MicroRNA-760 and Upregulating Hepatoma-Derived Growth Factor
Article Snippet: A normal human cervix epithelial cell line (Ect1/E6E7) was acquired from the American Type Culture Collection (Manassas, VA, USA).. Four human cervical cancer cell lines (HeLa, C-33A, SiHa, and CaSki) were ordered from the Shanghai Institute of Biochemistry and Cell Biology (Shanghai, China).Four human cervical cancer cell lines (HeLa, C-33A, SiHa, and CaSki) were ordered from the Shanghai Institute of Biochemistry and Cell Biology (Shanghai, China).
Article Title: Long Noncoding RNA SNHG7, a Molecular Sponge for microRNA-485, Promotes the Aggressive Behavior of Cervical Cancer by Regulating PAK4
Article Snippet: Four human cervical cancer cell lines (HeLa, SiHa, C-33A, and CaSki) were obtained from the Shanghai Institute of Cell Biology, the Chinese Academy of Sciences (Shanghai, China).Four human cervical cancer cell lines (HeLa, SiHa, C-33A, and CaSki) were obtained from the Shanghai Institute of Cell Biology, the Chinese Academy of Sciences (Shanghai, China).. Ect1/E6E7, a normal human cervix epithelial cell line, was purchased from the American Type Culture Collection (Manassas, VA, USA).. All cells were grown in Dulbecco’s modified Eagle’s medium (DMEM; Gibco; Thermo Fisher Scientific, Inc., Waltham, MA, USA) supplemented with 10% (v/v) of fetal bovine serum (FBS; Gibco; Thermo Fisher Scientific, Inc.) and 1% (v/v) of a solution of antibiotics (final concentration 100 U/mL penicillin and 100 μg/mL streptomycin; Gibco; Thermo Fisher Scientific, Inc.).All cells were grown in Dulbecco’s modified Eagle’s medium (DMEM; Gibco; Thermo Fisher Scientific, Inc., Waltham, MA, USA) supplemented with 10% (v/v) of fetal bovine serum (FBS; Gibco; Thermo Fisher Scientific, Inc.) and 1% (v/v) of a solution of antibiotics (final concentration 100 U/mL penicillin and 100 μg/mL streptomycin; Gibco; Thermo Fisher Scientific,..
Article Title: Long Noncoding RNA ZFPM2-AS1 Enhances the Malignancy of Cervical Cancer by Functioning as a Molecular Sponge of microRNA-511-3p and Consequently Increasing FGFR2 Expression
Article Snippet: Four cervical cancer cell lines, HeLa, SiHa, C-33A, and CaSki, were purchased from the Institute of Biochemistry and Cell Biology of the Chinese Academy of Sciences (Shanghai, China), and a normal human cervix epithelial cell line (Ect1/E6E7) from the American Type Culture Collection (Manassas, VA, USA).. All the aforementioned cell lines were maintained in Dulbecco’s modified Eagle’s medium (DMEM; Gibco; Thermo Fisher Scientific, Inc., Waltham, MA, USA) containing 10% of fetal bovine serum (FBS; Gibco; Thermo Fisher Scientific, Inc.), 100 U/mL penicillin, and 100 μg/mL streptomycin (Gibco; Thermo Fisher Scientific, Inc.) at 37 °C in a humidified atmosphere supplied with 5% of CO 2 .All the aforementioned cell lines were maintained in Dulbecco’s modified Eagle’s medium (DMEM; Gibco; Thermo Fisher Scientific, Inc., Waltham, MA, USA) containing 10% of fetal bovine serum (FBS; Gibco; Thermo Fisher Scientific, Inc.), 100 U/mL penicillin, and 100 μg/mL streptomycin (Gibco;..
Article Title: <p>Long Noncoding RNA SNHG7, a Molecular Sponge for microRNA-485, Promotes the Aggressive Behavior of Cervical Cancer by Regulating PAK4</p>
Article Snippet: Cell Lines Four human cervical cancer cell lines (HeLa, SiHa, C-33A, and CaSki) were obtained from the Shanghai Institute of Cell Biology, the Chinese Academy of Sciences (Shanghai, China).Cell Lines Four human cervical cancer cell lines (HeLa, SiHa, C-33A, and CaSki) were obtained from the Shanghai Institute of Cell Biology, the Chinese Academy of Sciences (Shanghai, China).. Ect1/E6E7, a normal human cervix epithelial cell line, was purchased from the American Type Culture Collection (Manassas, VA, USA).. All cells were grown in Dulbecco’s modified Eagle’s medium (DMEM; Gibco; Thermo Fisher Scientific, Inc., Waltham, MA, USA) supplemented with 10% (v/v) of fetal bovine serum (FBS; Gibco; Thermo Fisher Scientific, Inc.) and 1% (v/v) of a solution of antibiotics (final concentration 100 U/mL penicillin and 100 μg/mL streptomycin; Gibco; Thermo Fisher Scientific, Inc.).All cells were grown in Dulbecco’s modified Eagle’s medium (DMEM; Gibco; Thermo Fisher Scientific, Inc., Waltham, MA, USA) supplemented with 10% (v/v) of fetal bovine serum (FBS; Gibco; Thermo Fisher Scientific, Inc.) and 1% (v/v) of a solution of antibiotics (final concentration 100 U/mL penicillin and 100 μg/mL streptomycin; Gibco; Thermo Fisher Scientific,..
Article Title: <p>Long Noncoding RNA <em>ZFPM2-AS1</em> Enhances the Malignancy of Cervical Cancer by Functioning as a Molecular Sponge of microRNA-511-3p and Consequently Increasing FGFR2 Expression</p>
Article Snippet: The obtained tissue specimens were quickly frozen in liquid nitrogen and stored in liquid nitrogen until analysis.The obtained tissue specimens were quickly frozen in liquid nitrogen and stored in liquid nitrogen until analysis.. Cell Lines Four cervical cancer cell lines, HeLa, SiHa, C-33A, and CaSki, were purchased from the Institute of Biochemistry and Cell Biology of the Chinese Academy of Sciences (Shanghai, China), and a normal human cervix epithelial cell line (Ect1/E6E7) from the American Type Culture Collection (Manassas, VA, USA).. All the aforementioned cell lines were maintained in Dulbecco’s modified Eagle’s medium (DMEM; Gibco; Thermo Fisher Scientific, Inc., Waltham, MA, USA) containing 10% of fetal bovine serum (FBS; Gibco; Thermo Fisher Scientific, Inc.), 100 U/mL penicillin, and 100 μg/mL streptomycin (Gibco; Thermo Fisher Scientific, Inc.) at 37 °C in a humidified atmosphere supplied with 5% of CO2.All the aforementioned cell lines were maintained in Dulbecco’s modified Eagle’s medium (DMEM; Gibco; Thermo Fisher Scientific, Inc., Waltham, MA, USA) containing 10% of fetal bovine serum (FBS; Gibco; Thermo Fisher Scientific, Inc.), 100 U/mL penicillin, and 100 μg/mL streptomycin..
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![Comparison of tetraspanin composition and characteristics of sEV derived from different cancer cells. SEV derived from <t>HeLa</t> cells ( A – C ), HepG2 cells ( D – F ), H1650 cells ( G – I ) and SH-SY5Y cells ( J – L ) were captured using specific antibody-coated spots against CD63, CD81, CD9 and analyzed using the ExoView R100 platform. ( A , D , G , J ) Size distribution of cancer cell-derived sEV on tetraspanin capture spots analyzed using the SP-IRIS mode of the ExoView R100 platform. Box and Whisker Plot depicts the size distribution of all measured sEV on their respective capture spots. Box indicates the 25 to 50 percentile range and whiskers indicate the 5 to 95 percentile range. ( B , E , H , K ) Total fluorescent particle count of cancer cell-derived sEV on tetraspanin capture spots and mouse IgG isotype control analyzed using fluorescent antibodies against CD9/CD63/CD81. Plotted is the mean ± SEM of three independent biological experiments with three technical replicates, respectively. Single measurements are indicated as dots ( C , F , I , L ) Tetraspanin colocalization (coloc) of cancer cell-derived sEV using three fluorescent channels and overlay of fluorescent images. Data shown represents the respective tetraspanin colocalization fraction [%] out of all detected sEV. Plotted is the mean of three independent biological with three technical replicates, respectively.](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_9185/pmc09369185/pmc09369185__ijms-23-08544-g002.jpg)